BPCPPDYSOE21 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Bioimaging and Biospectroscopy
BP 8.4: Vortrag
Montag, 22. März 2021, 15:00–15:20, BPb
Dissection of Plasmodium falciparum developmental stages with multiple imaging methods — •Katharina Preißinger1,2, Beáta Vértessy1,2, István Készmárki3,4, and Miklós Kellermayer5 — 1Department of Applied Biotechnology and Food Sciences, BME, Budapest, Hungary — 2Institute of Enzymology, Research Center for Natural Sciences, Budapest, Hungary — 3Department of Physics, BME, Budapest, Hungary — 4Department of Experimental Physics V, University of Augsburg, Germany — 5Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
Efficient malaria treatment is a global challenge, requiring in-depth insight into the maturation of malaria parasites during the intraerythrocytic cycle. Exploring structural and functional variations of the parasites and their impact on red blood cells (RBCs) is a cornerstone of antimalarial drug development. In order to trace such changes in fine steps of parasite development, we performed an imaging study of RBCs infected by Plasmodium falciparum, using atomic force microscopy (AFM) and total internal reflection fluorescence microscopy (TIRF), further supplemented with bright field microscopy for the direct assignment of the stages. This multifaceted imaging approach allows to reveal correlations of the parasite maturation with morphological and fluorescence properties of the stages. We established identification patterns characteristic to the different parasite stages based on the height profile of infected RBCs which show close correlation with typical fluorescence (TIRF) maps of RBCs.