SKM 2021 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 4: Posters Biological Physics
BP 4.3: Poster
Donnerstag, 30. September 2021, 11:15–12:15, P
Optical Stretcher for Adherent Cells — •Alexander Janik1, Tobias Neckernuß1, Nathalie Neffgen2, Jonas Pfeil1, Mika Lindén2, and Othmar Marti1 — 1Institute of Experimental Physics, Ulm University — 2Institute of Inorganic Chemistry II, Ulm University
We have demonstrated a method to stretch adherent cells with a parallel laser beam to probe their mechanical properties. This contribution focuses on improvements of the setup as well as on interactions between cells and the illumination light. Progress has recently been made in the detection of the z position of the upper cell membrane, which is now achieved by tracking fluorescent beads on the cell. This yields a high z resolution and eliminates artefacts resulting from laser induced aberrations, which affect mainly detection rays entering the objective at small angles.