SKM 2021 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 4: Posters Biological Physics
BP 4.8: Poster
Donnerstag, 30. September 2021, 11:15–12:15, P
Bio-inspired Magnetic Nanoprobes For Subcellular Manipulation Studies in Single Cells — •Andreas Neusch1, Iuliia Novoselova1, Julia-Sarita Brand1, Marius Otten1, Matthias Karg1, Michael Farle2, Ulf Wiedwald2, and Cornelia Monzel1 — 1Heinrich-Heine-University, Düsseldorf — 2University of Duisburg-Essen, Duisburg
Cellular signals rely on characteristic temporal and spatial distributions of signaling molecules, but hitherto it is unclear which patterns trigger which cellular response. In recent years, Magnetogenetics emerged as an approach where magnetic nanoparticles (MNPs) and magnetic fields are used to spatially manipulate molecules to trigger cellular processes in order to mimic and study natural signaling patterns [Monzel et al. (2017), DOI: 10.1039/C7SC01462G]. Here, we compared two MNPs regarding their use as nanoagents of cellular functions. First, a bio-inspired semisynthetic nanoparticle - Magnetoferritin (MFt) - was chosen, which consists of the iron storage protein ferritin and a synthetic magnetic iron oxide core. MFt is genetically equipped with mEGFP for microscopic observation and bio-orthogonal targeting [Lisse et al. (2017), DOI: 10.1002/adma.201700189]. Furthermore, synthetic iron-oxide MNPs (synomag, micromod, Rostock) were studied. After examining basic properties, we assessed methods of transfer into cells and probed MNP manipulation in the cytoplasm. Using external magnetic fields, MNPs were spatially redistributed and kinetically analyzed. Our magnetic manipulation approach bears the perspective to achieve an understanding of how cell signals evolve.