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SKM 2021 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 8: Poster Session III

CPP 8.6: Poster

Mittwoch, 29. September 2021, 17:30–19:30, P

Protein-polysaccharide nanoparticles stabilized by thermal treatment — •Johannes Allwang1, Yanan Li1, Stefano Da Vela2, Dimitris Selianitis3, Angeliki Chroni3, Aristeidis Papagiannopoulos3, and Christine M. Papadakis11Physics Department, Technica University Munich, Garching, Germany — 2EMBL, DESY, Hamburg, Germany — 3Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, Athens, Greece

Protein complexes appear in healthy and diseased life forms as well as in food systems and medical applications. Their study can, therefore, give vital insights into biological mechanisms and help understand and treat diseases. Thermal treatment of protein-polysaccharide complexes was shown to create tunable nanoparticles with potential applications as carriers for nutriens and drugs [1].

Here, we investigate nanoparticles from trypsin; a proteolytic enzyme from the small intestine [2]. We use two different polysaccharides: hyaluronic acid and chondroitin sulfate. Synchrotron small-angle x-ray scattering is combined with light scattering methods to get insight into both the internal structure of the particles and their overall size.

[1] A. Papagiannopoulos, E. Vlassi, A. Radulescu, Carbohydr. Polym. 2019, 218, 218

[2] S. Trampari, A. Papagiannopoulos, S. Pispas, Biochem. Biophys. Res. Commun. 2019, 515, 282

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