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BP: Fachverband Biologische Physik
BP 7: Poster 1
BP 7.10: Poster
Monday, September 5, 2022, 18:00–20:00, P1
The effect of D2O on the pressure dependent protein-protein interaction in aqueous lysozyme solutions — •Michelle Dargasz, Jaqueline Savelkouls, and Michael Paulus — Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany
In some experimental techniques such as neutron scattering, the substitution of H2O with D2O is used to obtain a useful signal. It was assumed that the exchange of the solvent does not have a major influence on the protein structure and interactions. However, measurements with lysozyme in D2O revealed a larger attractive component of the protein-protein interaction potential [1]. In this study, the pressure-dependent behavior of high concentrated lysozyme solutions with H2O and D2O was considered using SAXS at the beamline BL2 of the synchrotron radiation source DELTA (Dortmund Germany). In previous measurements a non-linear relationship between the interaction potential and the exerted pressure was observed [2]. This was also visible in this study, as a shift in the correlation peak of the scattering curve occurs as a function of pressure. Up to approx. 2 kbar, a shift to larger q-values occurs, which is reversed with further increasing pressure up to 4 kbar. Since this effect occurs equally in H2O as well as D2O, it can be assumed that the water structure plays a rather minor role for the nonlinear correlation.
[1] C. Gripon, Journal of Crystal Growth 178, 575-584 (1997)
[2] Martin A. Schroer, Phys. Rev. Lett. 106, 178102 (2011)