Regensburg 2022 – scientific programme

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BP: Fachverband Biologische Physik

BP 9: Bioimaging

BP 9.1: Talk

Tuesday, September 6, 2022, 09:30–09:45, H16

Nano-infrared spectroscopic imaging (NanIRim): Promises and Challenges for Application in Biophotonics — •Daniela Täuber — Leibniz Institute of Photonic Technology, Jena, Germany — Friedrich-Schiller University Jena, Germany

A number of nano IR spectroscopic methods have been developed, which provide chemical information at subcellular and single molecule level. High spatial resolution leads to a reduction of the number of chemical bonds contributing to the signal. Thus, variations have to be identified above a heterogeneous background. Since 2019, I have investigated advantages and limitations of mid IR photo-induced force microscopy (PIF-IR) together with my team and collaborators. We applied PIF-IR to materials ranging from organic monolayers on various substrates, and biopolymer compositions to single bacteria and human retina. Recently, we studied interference effects in layered systems comparing experimental and calculated FTIR spectra of polymer films on different substrates to PIF-IR spectra. PIF-IR enables hyperspectral imaging at a fascinating spatial resolution of ~5 nm. A drawback are the small data sets. We applied PIF-IR to well-known interactions of antibiotics with Bacillus subtilis. To meet the challenge of finding the local interactions in hyperspectral images of single bacteria, we developed an advanced cluster analysis together with colleagues in the Heintzmann Lab. Our findings are very promising for successful applications of PIF-IR to the investigation of local variations in the surface areas of cells and tissues. Such visualization at the single cell level will boost our understanding of interactions in the Life Sciences