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BP: Fachverband Biologische Physik
BP 15: Tissue Mechanics II
BP 15.3: Vortrag
Mittwoch, 29. März 2023, 11:00–11:15, BAR Schö
Viscoelastic measurements in glioblastoma-infiltrated cerebral organoids — •Michael Frischmann1, Elijah Shelton1, Sofia Kalpazidou2, Jovica Ninkovic2,3, and Friedhelm Serwane1,3 — 1Faculty of Physics and Center for NanoScience, LMU Munich, Germany — 2Biomedical Center, LMU Munich, Germany — 3Munich Cluster for Systems Neurology, Munich, Germany
The glioblastoma is a malignant neuroepithelial brain tumor with median survival rates of a few months without treatment. One reason is the rapid infiltrative growth with active destruction of brain tissue and the resulting necrotic debris. Glioblastomas are well described from a molecular biology perspective. However, little is known about their mechanical properties which directly affect the tumor’s ability to spread into adjacent tissues. I will present measurements of mechancial properties of glioblastoma and its surrounding tissue. To study tumor biophysics in an accessible in vitro system, we use cerebral organoids grown from induced pluripotent stem cells (iPSCs) with implantet glioblastoma cells. To determine the mechancial properties of the tumor and its microenvironment, we use magnetic microdroplets that we inject into the tissue via microneedles. Actuated by a homogeneous magnetic field, the droplet deforms and its deformation is recorded via a confocal microscope. The dynamic deformation is used to infer the viscoelastic properties of physiological and pathological tissue, as well as boundary regions. The recorded data allows us to establish a viscoelastic model of glioblastoma and to develop a mechanical understanding how brain tumors infiltrate their environment.