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BP: Fachverband Biologische Physik

BP 17: Protein Structure and Dynamics

BP 17.9: Vortrag

Mittwoch, 29. März 2023, 17:15–17:30, BAR 0106

X-ray damage to Gene-V Protein: NAP-XPS analysis of Chemical changes to Proteins in Water — •Dorothea C Hallier^1,2,3, Jörg Radnik², Paul M Dietrich⁴, Harald Seitz^1,3, and Marc Benjamin Hahn² — ¹Fraunhofer Insitute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany — ²Federal Insitute for Materials Research and Testing BAM Berlin, Berlin, Germany — ³Univerity of Potsdam, Institute for Biochemistry and Biology, Potsdam Germany — ⁴SPECS Surface Nano Analysis GmbH, Berlin, Germany

X-ray photoelectron spectroscopy (XPS) was used to analyze the chemical damage of ionizing radiation to a single-stranded DNA-binding protein: Gene-V Protein (G5P/GVP) and its most abundant amino acids (Alanine, Arginine, Cysteine, Glycine, Lysine, Methionine, Tyrosine). This protein plays a crucial role in maintaining the DNA metabolism, especially DNA replication, recombination and repair. Vacuum measurements were combined with near-ambient pressure (NAP) XPS measurements under water and nitrogen atmosphere to detect both direct and indirect radiation damage and corresponding damage pathways. The exposure of proteins and aminoacids to x-rays leads to degradation i.e. via dehydrogenation, decarboxylation, dehydration and deamination. A strong increase of protein damage was observed in water as compared to vacuum.