SKM 2023 – scientific programme
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BP: Fachverband Biologische Physik
BP 17: Protein Structure and Dynamics
BP 17.9: Talk
Wednesday, March 29, 2023, 17:15–17:30, BAR 0106
X-ray damage to Gene-V Protein: NAP-XPS analysis of Chemical changes to Proteins in Water — •Dorothea C Hallier1,2,3, Jörg Radnik2, Paul M Dietrich4, Harald Seitz1,3, and Marc Benjamin Hahn2 — 1Fraunhofer Insitute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany — 2Federal Insitute for Materials Research and Testing BAM Berlin, Berlin, Germany — 3Univerity of Potsdam, Institute for Biochemistry and Biology, Potsdam Germany — 4SPECS Surface Nano Analysis GmbH, Berlin, Germany
X-ray photoelectron spectroscopy (XPS) was used to analyze the chemical damage of ionizing radiation to a single-stranded DNA-binding protein: Gene-V Protein (G5P/GVP) and its most abundant amino acids (Alanine, Arginine, Cysteine, Glycine, Lysine, Methionine, Tyrosine). This protein plays a crucial role in maintaining the DNA metabolism, especially DNA replication, recombination and repair. Vacuum measurements were combined with near-ambient pressure (NAP) XPS measurements under water and nitrogen atmosphere to detect both direct and indirect radiation damage and corresponding damage pathways. The exposure of proteins and aminoacids to x-rays leads to degradation i.e. via dehydrogenation, decarboxylation, dehydration and deamination. A strong increase of protein damage was observed in water as compared to vacuum.