SKM 2023 – wissenschaftliches Programm

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DY: Fachverband Dynamik und Statistische Physik

DY 35: Statistical Physics of Biological Systems I (joint session BP/DY)

DY 35.11: Vortrag

Donnerstag, 30. März 2023, 12:30–12:45, TOE 317

Hydrationa and crowding effects on SOD1 sequestration into FUS biocondensatesLuis Enrique Coronas1, Emeline Laborie2, Stepan Timr2, Fabio Sterpone2, and •Giancarlo Franzese1, 31Interdisciplinary and Statistical Physics Section--Department of Condensed Matter Physics, Physics & Institute of Nanoscience and Nanotechnology (IN2UB), Universitat de Barcelona, Barcelona, Spain — 2CNRS Laboratoire de Biochimie Théorique, Institut de Biologie Physico-Chimique, Université Paris Denis Diderot, Paris, France — 3Max Planck Institut für Physik Komplexer Systeme, Dresden, Germany

Superoxide Dismutase 1 (SOD1) is a protein related to amyotrophic lateral sclerosis that, under Heat Stress (HS), is sequestered into Stress Granules in vivo and Fused in Sarcoma (FUS) biomolecular condensates in vitro. Experiments show that an in vitro cytomimetic medium, using Bovine Serum Albumin (BSA) as crowder, decreases the SOD1 partition coefficient (PC) even after 60 min of HS. Implicit-water OPEP simulations show no preferential interactions of SOD1 with BSA. Here, by combining the OPEP with a coarse-grained water model, accounting for water contributions to the interactions in large biological systems, we show that SOD1 has one preferred associative state in FUS but three in BSA, whose transition rates and residency times are controlled by their hydration. We conclude that the SOD1 PC's decrease in FUS condensate, when BSA crowders are present, is due to the hydration entropy increase in BSA, a mechanism possibly relevant also in vivo.

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