Dresden 2026 – scientific programme
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BP: Fachverband Biologische Physik
BP 14: Poster Session II
BP 14.30: Poster
Tuesday, March 10, 2026, 18:00–21:00, P2
Multimodal imaging with light microscopes and scanning small angle X-ray scattering — •Boram Yu1, Mangalika Sinha1, Rita Mendes da Silva1,2, Ulrike Rölleke1, Manfred Burghammer2, and Sarah Köster1 — 1Institute for X-Ray Physics, University of Göttingen, Germany — 2The European Synchrotron, Grenoble, France
Imaging biological cells using X-rays is a complementary approach to electron and fluorescence microscopy due to their high penetration depth and the possibility for label-free imaging. One such technique is scanning small angle X-ray scattering (SAXS), which enables the acquisition of a real-space overview image with moderate resolution and reciprocal-space information with high resolution of cells in aqueous environment. However, intracellular structures in aqueous environment are very sensitive to radiation; therefore, the investigation of such damage requires additional imaging techniques beyond scanning SAXS. We present a scanning SAXS configuration, which integrates a mobile fluorescence microscope and an on-axis bright field microscope with a microfluidic sample chamber. The microfluidic sample chamber is compatible with X-ray measurement, as well as two microscopes, owing to its very thin design. While the fluorescence microscope enables obtaining correlative images before and after X-ray scanning within a short time span, the bright field microscope produces bright field images simultaneously during the scan. Our approach provides complementary information on specimens to X-ray measurements, thereby demonstrating its applicability in various fields.
Keywords: X-ray imaging; small angle X-ray scattering; microscopy; multimodal imaging