Dresden 2026 – scientific programme
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BP: Fachverband Biologische Physik
BP 14: Poster Session II
BP 14.85: Poster
Tuesday, March 10, 2026, 18:00–21:00, P2
Dynamics of tissue sampling of resident tissue macrophages — •Miriam Schnitzerlein1, 2, Eric Greto3, 4, Stefan Uderhardt3, 4, and Vasily Zaburdaev1, 2 — 1Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen — 2Max-Planck-Zentrum für Physik und Medizin, Erlangen — 3FAU und Universitätsklinikum Erlangen — 4Optical Imaging Competence Center Erlangen, FAU
Resident tissue macrophages (RTMs) are a type of immune cell present in essentially every tissue in the human body. One of their main functions is to keep the tissue in homeostasis by resolving lesions or removing dead cells, thereby preventing unnecessary inflammation and avoiding collateral damage to the tissue. To find such incidents, RTMs show continuous sampling behaviour by extending and retracting cell protrusions. This sampling behaviour needs to be tightly regulated - due to finite amounts of available cell cytoplasm and membrane - while still guarding the entirety of the tissue.
In this project, we have employed a high-resolution, intravital imaging protocol to generate dynamic data of murine RTMs in vivo in the peritoneum. Next we have built a custom image processing pipeline to segment RTM protrusions and their dynamic behaviour. We could then analyse the sampling range of protrusions and found correlations between outgrowth and shrinking of protrusions. Furthermore, the data hints at a division of labour approach protrusions employ when scanning the tissue.
Keywords: Image analysis; Resident tissue macrophages; Dynamics of tissue sampling; Correlations of protrusion growth