Dresden 2026 – scientific programme
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BP: Fachverband Biologische Physik
BP 33: Bioimaging
BP 33.9: Talk
Thursday, March 12, 2026, 17:30–17:45, BAR/0205
Effects of Optical Stimulation on the Adhesion of Human Osteoblasts — •Franziska Dorn1, Wiebke Wollenberg2, Meike Bielfeldt3, Regina Lange1, Susanne Stählke3, Ingo Barke1, Henrike Rebl3, Berit Zeller-Pulmhoff2, and Sylvia Speller1 — 1Physics of Surfaces and Interfaces, University Rostock — 2Data-Driven Analysis and Design of Materials, University of Rostock — 3Institute of Cell Biology, University Medical Center Rostock
Accelerated growth of autologous bone tissue is a promising strategy in regenerative medicine. In this study, we investigated how optical stimulation influences cell adhesion. We cultured the osteoblast-like (MG-63) cells in fetal bovine serum-free physiologic medium. As substrates, we used plain glass and glass surfaces with structured gold nanotriangular islands fabricated by nanosphere lithography. After cell seeding, the samples were illuminated with green light, inducing a spatially varying light intensity due to reflection and plasmons. Afterwards, we examined the cells using scanning electron microscopy and applied machine-learning-based/random-forest-based segmentation to extract morphological features. We found that opto-stimulated cells form more concave borders than unstimulated controls, suggesting that the cytoskeleton and the focal adhesions develop at non-uniform speed. Additionally, the adhesion area of illuminated cells is substantially larger than that of control cells on glass surfaces. However, when cells are seeded on surfaces with gold nanotriangular islands, this effect is absent. In a next step the peripheral region of the cells, in terms of lamellipodia and filopodia is addressed. (SFB 1270-299150580)
Keywords: Osteoblasts; Opto-Stimulation; Scanning Electron Microscope (SEM); Machine Learning (ML); Nanosphere Lithography