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SYC: Symposium Einzelne Atome, Ionen und Moleküle
SYC 4: Poster Symposium Einzelne Atome, Ionen und Moleküle
SYC 4.5: Poster
Mittwoch, 18. März 1998, 16:00–18:00, PF
Low Temperature Confocal Scanning Optical Microscopy of single Molecules — •Jean-Manuel Segura1, Bert Hecht2, Alois Renn2, and Urs P. Wild2 — 1Physical Chemistry LaboratorySwiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zurich, Switzerland — 2Swiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zurich, Switzerland
Single molecules in solid matrices at cryogenic temperature exhibit extremely narrow zero phonon lines whose width and spectral position are extremely sensitive to local variations in the environment. In order to assign spectral behavior to structures in the molecular environment we use fluorescence confocal scanning optical microscopy (CSOM). CSOM provides highly resolved three dimensional images and allows also for spatial selection and spectral investigation of appropriately chosen single molecules. A bimorph piezo scanner and a stepper motor driven microscope objective reside in a cryostat that can be optically accessed via a bottom window and thus allows for an exceptionally large sample volume. Exploiting the large space, the bimorph scanner has been designed to have a scan range of 20 µ even at superfluid helium temperature. CSOM images of single molecules at low temperature will be presented.