Berlin 2001 – wissenschaftliches Programm
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CPP: Chemische Physik und Polymerphysik
CPP 17: Poster: Spectroscopy and Single Particle Spectroscopy of Molecular Systems, Photoprocesses, Biological Systems
CPP 17.25: Poster
Dienstag, 3. April 2001, 12:30–15:00, AT1
Investigation on the TRAF2–Signaling Cascade — •Elmar Thews1, Andrew Aird1, Axel Wechsler1, Jan Exmann1, Fedor Jelezko1, Carsten Tietz1, Harald Wajant2, and Jörg Wrachtrup1 — 13. Physikalisches Institut, Universität Stuttgart, 70550 Stuttgart, Germany — 2Institut für Zellbiologie und Immunologie, Universität Stuttgart, 70550 Stuttgart , Germany
Cell signaling is one of the most promising research fields in single molecule spectroscopy. Among the processes requiring signal transduction, programmed cell death (apoptosis) and NFκB activation are two of the most important ones because of their relevance for cancer research. Besides membrane receptors, numerous intracellular signaling proteins are involved in these pathways. The No-dqTumor Necrose Factor (TNF) associated Factor 2No-dq (TRAF2) is the prototypic member of the TRAF protein family and critically involved in TNF-R1 induced activation of NFkB and cell death.
The poster focuses on the investigation of TRAF2–localization, conformation, and signal kinetics with several techniques. As the protein structure is known by x-ray diffraction, molecular dynamic (MD) simulations help resolving the conformation of the TRAF2-trimer with or without additionally attached Green Fluorescent Protein (GFP) mutants, used to label the trimer for fluorescent detection. Calculation of Fluorescence Resonant Energy Transfer (FRET) rates and subsequent comparison to measured ones in lysate reveals whether GFP attached trimers will be hindered in their binding kinetics to their receptor.