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Q: Quantenoptik

Q 30: Poster: Laser in Medicine

Q 30.5: Poster

Friday, April 6, 2001, 12:30–15:00, AT2

Detection of malignant lesions during endoscopy of the oesophagus by laser-induced delayed fluorescence spectroscopy — •Uwe Sukowski¹, Bernd Ebert¹, Marianne Ortner², Eike Hein², Katharina Zumbusch², Dirk Nolte¹, Manfred Dietel³, Herbert Lochs², and Herbert Rinneberg¹ — ¹Physikalisch-Technische Bundesanstalt, Abbestr. 2 - 12, 10587 Berlin — ²4th Medical Department, Medical Faculty Charite, Schumannstr. 20/21, 10117 Berlin — ³Department of Pathology, Medical Faculty Charite, Schumannstr. 20/21, 10117 Berlin

We have investigated whether laser-induced fluorescence can aid endoscopic detection of malignant lesions in Barrett’s oesophagus. Protoporphyrin IX (PpIX) accumulates in malignant tissue after aminolaevulinic acid was sprayed on the mucosa. PpIX fluorescence guided biopsies were taken. To this end delayed laser-induced fluorescence of PpIX was excited (λ = 505 nm) by the output of an OPO pumped by the 3^rd harmonic of a Nd:YAG laser. The same fiber was used to deliver the laser beam through the endoscope to the mucosa, and to collect fluorescence to be analyzed by an optical multichannel analyzer. From prompt and delayed fluorescence spectra of these biopsies recorded ex vivo normalized PpIX fluorescence intensities were derived and compared with histological results. For the first time we differentiated low-grade dysplasia from Barrett’s mucosa during endoscopy based on laser-induced fluorescence.