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Berlin 2001 – wissenschaftliches Programm

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Q: Quantenoptik

Q 30: Poster: Laser in Medicine

Q 30.5: Poster

Freitag, 6. April 2001, 12:30–15:00, AT2

Detection of malignant lesions during endoscopy of the oesophagus by laser-induced delayed fluorescence spectroscopy — •Uwe Sukowski1, Bernd Ebert1, Marianne Ortner2, Eike Hein2, Katharina Zumbusch2, Dirk Nolte1, Manfred Dietel3, Herbert Lochs2, and Herbert Rinneberg11Physikalisch-Technische Bundesanstalt, Abbestr. 2 - 12, 10587 Berlin — 24th Medical Department, Medical Faculty Charite, Schumannstr. 20/21, 10117 Berlin — 3Department of Pathology, Medical Faculty Charite, Schumannstr. 20/21, 10117 Berlin

We have investigated whether laser-induced fluorescence can aid endoscopic detection of malignant lesions in Barrett’s oesophagus. Protoporphyrin IX (PpIX) accumulates in malignant tissue after aminolaevulinic acid was sprayed on the mucosa. PpIX fluorescence guided biopsies were taken. To this end delayed laser-induced fluorescence of PpIX was excited (λ = 505 nm) by the output of an OPO pumped by the 3rd harmonic of a Nd:YAG laser. The same fiber was used to deliver the laser beam through the endoscope to the mucosa, and to collect fluorescence to be analyzed by an optical multichannel analyzer. From prompt and delayed fluorescence spectra of these biopsies recorded ex vivo normalized PpIX fluorescence intensities were derived and compared with histological results. For the first time we differentiated low-grade dysplasia from Barrett’s mucosa during endoscopy based on laser-induced fluorescence.

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