Regensburg 2002 – wissenschaftliches Programm
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SYBM: Physik biologischer Materie
SYBM 401: Physics of biological matter: Poster
SYBM 401.25: Poster
Dienstag, 12. März 2002, 18:00–19:30, H37
DIFFUSION PROPERTIES OF SINGLE LIPIDS AND CONNEXONES IN MEMBRANES OF LIVING CELLS — •Elmar Thews1, Axel Wechsler1, Jörg Wrachtrup1, and Reiner Eckert2 — 13. Physikalisches Institut der Universität Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart — 2Biologisches Institut der Universität Stuttgart Abteilung Biophysik, Pfaffenwaldring 57, 70569 Stuttgart
In this talk I present the results obtained in colaboration with the Biologisches Institut der Universität Stuttgart, Abteilung Biophysik. A Total Internal Reflection Fluorescence Microscope (TIRFM) was used to image the footprint, i.e. the cellmembrane close to the coverslip, of living cells under physiologic conditions. At first MDCK-cells were stained with a DiI-DOPC lipid in low concentration to be persued afterwards in time. In a second set of experiments HeLa-cells were transiently transfected to express the GFP-Connexine fusion protein, which, forming a hexamer (connexone), was detected mostly in aggregated oligomers. The recorded movies were evaluated (and some are shown) by a home-made software package to extract single trajectories and calculate Mean Square Displacement (MSD) plots. The calculation of the MSD plot from a single trajectory gives rise to a differentiation of the possible diffusion processes in cells: e.g. normal diffusion, anomalous diffusion, coralled diffusion or directed transport.