Regensburg 2002 – wissenschaftliches Programm
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SYBM: Physik biologischer Materie
SYBM IV: HV IV
SYBM IV.1: Hauptvortrag
Dienstag, 12. März 2002, 11:45–12:15, H37
Manipulating and Probing Cellular Properties of Fibroblasts and Melanocytes through Contact with Nanostructured Surfaces — •Joachim Spatz1, Ralf Kemkemer1, Alexandre Micoulet1, Roos Wouter1, and Dieter Kaufmann2 — 1Heidelberg University, Institute for Physical Chemistry, Biophysical Chemistry, INF 253, 69120 Heidelberg — 2Ulm University, Human Genetic, 89081 Ulm
Chemical-physical properties of synthetic macromolecules and peptides have been used to modify surfaces on the molecular level through nanopatterning. Biofunctionalisation of nanostructured, solid interfaces will be discussed. The surfaces are applied as a tool to investigate cluster formation of focal adhesion associated proteins of fibroblasts. Cultured human melanocytes were used in order to study the regulation of cell shape through contact with interfaces offering different topography and biochemical pattern. The cellular morphology of melanocytes is a measurable indicator for cell reaction to the cellular environment. The characteristic cellular shape of different cell cultures was quantified by different shape parameters like the number and length of dendrites. A decreased signal-to-noise ratio was found for melanocyte cells concerning the number of dendrites and orientation of dendrites if cultured on biochemically and topographically structured substrates.