München 2004 – wissenschaftliches Programm
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Q: Quantenoptik und Photonik
Q 43: Biophotonik und Laser in der Medizin
Q 43.8: Vortrag
Donnerstag, 25. März 2004, 18:15–18:30, HS 224
A multicolor confocal laser scanning microsope with fast spectral detection by CCD — •Joachim Walter1, Christian Seebacher2, and Rainer Uhl1,2 — 1Till I.D., Am Klopferspitz 19, 82152 Martinsried — 2Bioimaging Zentrum,m Am Klopferspitz 19, 82152 Martinsried
Confocal laser scanning microscopes are routinely used in biological research for the generation of 3D microscopical images of fluorescently tagged structures. Simultaneous imaging of multiple structures requires the ability to separately detect a large number of fluorochromes. Obstacles to the spectral separation are overlapping excitation and emission spectra of fluorochromes and limited detection efficiency of microscopes. We present a confocal microscope setup with increased detection efficiency. This is achieved by two approaches: First, a beamsplitter with higher edge steepness than common dichroic mirrors is employed. Second, a CCD camera is used as detector, which has a 2-10 times higher quantum efficiency than commonly used photomultipliers in the relevant spectral region. The CCD camera is part of a prism spectrograph. With this setup full spectra or flexibly binned spectral bands of the emission light can be recorded at timescales of microseconds.