Regensburg 2004 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 50: Poster Session "Biological Physics"
AKB 50.14: Poster
Freitag, 12. März 2004, 10:30–13:00, B
Dynamics of cell adhesion contacts and forces on rigid nanoadhesive templates — •Christine Selhuber1, Marco Arnold1, Roman Glass1, Jacques Blümmel1, Horst Kessler2, and Joachim Spatz1 — 1Universität Heidelberg, Biophysikalische Chemie, INF 253, 69120 Heidelberg — 2TU München, Institut für Organische Chemie und Biochemie, Lichtenbergstrasse 4 , 85747 Garching
Cooperative processes in cell adhesion are one of the most fundamental issues in cell sciences which control many cell functions. Nanometer sized RGD based adhesive dots of an extension smaller 8 nm are applied as bindin g sites for the activation of a single integrin per dot. The dots assemble with high precision on interfaces where the pattern geometry and the separation of single dots can be controlled in a flexible way. Thus, the pattern resembles a rigid adhesive template on which cell or vesicle adhesion can be probed with single receptor resolution. If adhesive dots are separated by more than 73 nm cell adhesion fails due to extended singl e integrin-integrin separation, as well as cell spreading, formation of foca l contact clusters and actin stress fibre formation is constricted. The adhesion forces generated on such substrates are measured as a function of nanopattern geometry and adhesion time. The dynamic change of the cell adhesive area is determined using reflection interference contrast microscopy.