Berlin 2005 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe

AKB: Biologische Physik

AKB 200: Poster Session II

AKB 200.15: Poster

Dienstag, 8. März 2005, 17:00–19:00, Poster TU C

Single cell manipulation in microfluidic networks by optical tweezers — •Kai Leffhalm, Andy Sischka, Wibke Hellmich, Thanh Tu Duong, Katja Tönsing, Robert Ros, Alexandra Ros, and Dario Anselmetti — Experimental Biophysics, Physics Department, Bielefeld University, Germany

Control and manipulation of single cells gain importance as a tool to better understand the migration behaviour of living cells in vivo and for single cell analysis. Microfluidic networks provide dimensions small enough to navigate and steer single cells with optical tweezers to different areas of an artificial network where the flow properties can be controlled by electrophoresis and electroosmosis.

Potential applications include microproteomics and monitoring of the expression level of individual cells, which can be stimulated or suppressed by changing the velocity of the flow or the concentration of substances, e.g. cytokines or (cytostatic) drugs, in the culture medium.

We will present our experimental setup and our first test experiments where a cell is captured between two electrodes where it can be destroyed by an electric field, i.e. an electric pulse. This is an initial point for future single cell analysis.