Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 200: Poster Session II

AKB 200.32: Poster

Dienstag, 8. März 2005, 17:00–19:00, Poster TU C

Towards a Combined Approach of Single Molecule Tracking and Fluorescence Correlation Spectroscopy — •Stephan Schäfer — Biotechnological Centre, TU Dresden

Wide-Field Microscopy based on CCD detection and tracking of single fluorescently labelled molecules (SMT) in lipid bilayer membranes has proved to be a promising tool for a deeper understanding of biologically active lipid- and protein molecules. However, tracking of single molecules with a high mobility remains difficult due to limitations in the camera read-out rate and signal collection time. Using giant unilamellar vesicles (GUVs) which have been proven to be a suitable and reliable model for biological lipid membranes restricting diffusive motion to their 2D membrane surface. Besides SMT we employes Fluorescence correlation spectroscopy (FCS) as a time-averaging fluctuation analysis of small molecular ensembles. Comprising maximum sensitivity and high statistical confidence, FCS is especially well suited for investigations within cellular membranes. We present first results of a comparative study on SMT and FCS performed on the lipid bilayer membrane of GUVs. There is strong indication of a purely Brownian diffusion for the investigated composition (50