Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 200: Poster Session II

AKB 200.34: Poster

Dienstag, 8. März 2005, 17:00–19:00, Poster TU C

Diamond-based biosensors — •Andreas Härtl¹, Jorge Hernando¹, Philipp Achatz¹, Tahmineh Pourrostami¹, Stefan Walter², Jose Garrido¹ und Martin Stutzmann¹ — ¹Walter Schottky Institut, Technische Universität München, Germany — ²Institut für Organische Chemie und Biochemie, Technische Universität München, Germany

Diamond is known to be a per se biocompatible material, consisting just of carbon atoms. Other interesting properties are a large electrochemical potential window, low thermal background currents, fouling resistance, and chemical inertness. Together with the existance of a quasi-two-dimensional conductive layer at the surface of hydrogen terminated diamond, this suggests the use of diamond as an active substrate which can interact with biomolecules immobilized on it.

In this contribution we report on the functionalization of diamond surfaces with various biomolecules and on diamond based ion sensitive field effect transistors (ISFET). We have studied the immobilization of different proteins on the surface of single crystal and nanocrystalline diamond substrates, showing that covalent bonding can be achieved. Moreover, we have confirmed that the immobilized proteins retain their biological activity. First amperometric sensor applications have been realized and will be presented.

We also have fabricated ISFETs on single crystalline and polycrystalline H-terminated diamond substrates and have investigated their pH sensitivity.