Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 200: Poster Session II
AKB 200.38: Poster
Dienstag, 8. März 2005, 17:00–19:00, Poster TU C
Scanning Probe Microscopy Investigations of the Oriented Attachment and Membrane Reconstitution of His-tagged Cytochrome c oxidase to a Gold Electrode — •Dirk Mayer1, Andreas Offenhäusser1, Kenichi Ataka2, and Joachim Heberle2 — 1Forschungszentrum Jülich, ISG-2: Institute for Bio and Chemosensors, Jülich, Germany — 2Forschungszentrum Jülich, IBI-2: Structural Biology, Jülich, Germany
Many of the vital functions of cells are maintained by membrane proteins, which for instance selectively control the transfer of ions, biological signal molecules and energ. The high complexity of biological transmembrane machineries with respect to their structure (consisting of many subunits), the reduced periodicity (scalablility) and the multistep reaction paths makes the assignment of structure and function a challenging task. Monolayers of reconstituted membrane proteins supported by solid surfaces can be applied to modern surface analyzing methods. We are giving an account of a novel approach, combining the modification of a metal surface by attaching a Ni- NTA moiety with the reconstitution of the oriented and detergent solubilized proteins in a lipid bilayer. We employed surface-enhanced infrared absorption spectroscopy and scanning probe techniques for deriving a detailed description of the whole solid surface supported immobilization and reconstitution reaction path.