Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 200: Poster Session II

AKB 200.40: Poster

Dienstag, 8. März 2005, 17:00–19:00, Poster TU C

Development of a biosensor device on functionalized Silicon on Insulator (SOI) structures for the specific detection of proteins — •Simon Lud, Cornelia Neunteufel, Petra Neff, Michael Nikolaides, M. Fischer, and Andreas Bausch — Lehrstuhl für Biophysik E22, TU München, 85747 Garching, Germany

To handle the vast number of possible interactions between different molecules, detection systems must be able to screen many different interactions in parallel. Further on, in order to account for the different physical properties of the involved molecules, the detector must have a tunable sensitivity.

We present a device based on standard semiconductor technology which enables the selective and quantitative detection of biomolecular interactions. The sensor is based on hydrophobized Silicon-On-Insulator (SOI) substrates and is functionalized by a monolayer of lipids with incorporated metal chelate lipids. Both reversible charging of the chelate headgroup with divalent nickel ions and the specific binding of proteins was detected In addition, it was possible to detect charge differences between both peptides and proteins quantitatively. The sensor response is modelled within the standard Poisson-Boltzman theory and thus an average effective charge of different peptides and proteins can be determined. As the device is based on standard semiconductor technologies the SOI based Biosensor is well suited for parallelization needed in high throughput applications.