Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 200: Poster Session II
AKB 200.66: Poster
Dienstag, 8. März 2005, 17:00–19:00, Poster TU C
Design of functional carbohydrate clusters for control of dynamic cell adhesion — •Jochen Oelke1, Andreea Banu1, Richard R. Schmidt2, Achim Wixforth3, and Motomu Tanaka1 — 1Dept. Phys. E22, TU München, D-85748 Garching, e-mail: mtanaka@ph.tum.de — 2Dept. Chem., Univ. Konstanz, D-78457 Konstanz — 3Dept. Phys., Univ. Augsburg, D-86135 Augsburg
The general goal of this work is the design of functional micro- and nano-clusters of ligands through controlled self-assembling processes at the interface, and their use as a new platform to control adhesion of bacteria under defined shear stresses.
As functional ligands, we synthesize a new class of fluorinated glycolipids bearing mannose or gal(1→4)-α-gal moieties. The fluorocarbon segment is used in order to induce de-mixinig due to the immicibility with the hydrocarbon segment, whereas the sugar part is responsible for the recognition with bacteria (for example mannose for enteroaggregative bacteria). As the first step, formation of these clusters was studied at the air/water interface, followed by deposition onto solid surfaces. The strength of bacterial adhesion, we determine the critical shear field required for the cell detachment (dissociation rate) by coupling the functionalized surface to two micro-fluidic systems: (a) conventional capillary flow chambers, and (b) No-dqflat-fluidicsNo-dq using surface acoustic waves (SAWs).