Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 200: Poster Session II

AKB 200.70: Poster

Dienstag, 8. März 2005, 17:00–19:00, Poster TU C

Acute Brain Slices on Silicon Chips: From Capacitive Stimulation to Recording with Field Effect Transistor Array — •Christian Stangl and Peter Fromherz — Max Planck Institute of Biochemistry, Dept. Membrane- and Neurophysics, Am Klopferspitz 18, D-82152 Martinsried

Silicon chips with arrays of capacitive stimulators and field effect transistors provide a novel approach in investigating the brain. Previous studies demonstrated the non-invasive stimulation and recording on cultured brain slices. Now for the first time acutely dissected brain slices have been used to record evoked neuronal field potentials.

Dead cell layers on the surface of acute slices, caused by the cutting procedure, complicate both capacitive stimulation and coupling with transistors. We treated this problem by simulating the distribution of evoked field potentials within the slice. The calculated profiles fit to data from conventional extracellular microelectrode measurements.

Capacitive stimulation and recording with field effect transistors are possible with the use of acute brain slices in spite of the dead cell layers. With this novel non-invasive approach we could probe neuronal projections in hippocampal slices as well as the neuronal plasticity of the hippocampus.