Berlin 2005 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe

AKB: Biologische Physik

AKB 25: Active Networks and Cell Motility

AKB 25.7: Vortrag

Freitag, 4. März 2005, 17:00–17:15, TU H2013

Resolving the Cell Cycle by Measuring Optical Deformability with a Microfluidic Optical Stretcher — •Bryan Lincoln, Maren Romeyke, Frank Sauer, Stefan Schinkinger, Falk Wottawah, and Jochen Guck — Universität Leipzig, Abteilung Physik der Weichen Materie, Linnéstr. 5, 04103 Leipzig

The Optical Stretcher is a dual-beam optical trap capable of measuring the deformability of suspended biological cells. Previous measurements with this system have shown relevant differences between various cell types and have supported the idea that there is a strong correlation between a cell’s cytoskeletal structure and its deformability. A microfluidic delivery system has been developed in order to measure cells at a high enough rate to acquire the necessary statistics to resolve elasticity changes within a single cell type. The cytoskeleton reorganizes as a cell grows and divides, so we find changes in deformability during various phases of the cell cycle as well. These various stages are identified by quantitative single-cell fluorescent DNA analysis. Results are presented using a three-element viscoelastic model and implications for deformability as a cell marker are discussed. Additional experiments using Laser Scanning Cytometry are presented that correlate position in the cell cycle with amount of F-actin in both the adhered and suspended state.