# Dresden 2006 – wissenschaftliches Programm

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# DY: Dynamik und Statistische Physik

## DY 41: Dynamical Physics in Biological Systems

### DY 41.2: Vortrag

### Donnerstag, 30. März 2006, 10:15–10:30, SCH 251

**A Bayesian Approach to the Evaluation of Dynamic Force Spectroscopy Experiments** — •Sebastian Getfert, Peter Reimann, and Martin Raible — Condensed Matter Theory, Universität Bielefeld, Universitätsstraße 25, 33615 Bielefeld

The components of a biomolecular complex can be connected via suitable linkers
to the tip of an atomic force microscope and a moving surface. When this surface
is pulled apart at constant velocity the force *f* that acts on the bond
increases (approximately) linearly in time until the chemical bond ruptures.
This process is of stochastic nature. The distribution of the rupture forces
and in particular the maximum depends in a characteristic way on the loading
rate ḟ and on binding parameters like dissociation length and
(force-free) dissociation rate [1]. Thus the evaluation of rupture force data
from dynamic force spectroscopy experiments allows to draw conclusions about the energy landscape of the bond. We present a Bayesian approach to this evaluation
in which experimental uncertainties can be included in a natural way. Further we discuss from a statistical point of view to which degree of accuracy the parameters of the distribution can be determined.

[1] E. Evans and K. Ritchie, Biophys. J. 72, 1541 (1997)