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ST: Strahlen- und Medizinphysik

ST 12: Biophysikalische Nanoskopie

ST 12.1: Invited Talk

Wednesday, March 15, 2006, 10:00–10:30, D

Laseroptical Nanoscopy: New Tools for Single Cell Analysis — •Christoph Cremer — Kirchhoff-Institut für Physik Universität Heidelberg, Im Neuenheimer Feld 227, D-69120 Heidelberg

For analytical pathology, a serious problem for the extension of *large scale* and *mesoscale* fluorescence image analyses of cellular structures to cellular nanostructures is the light microscopical resolution conventionally limited to about 200 nm laterally and 600 nm axially. In recent years, various laseroptical *nanoscopy* approaches to overcome this impasse have been developed. In this overview, optical principles and application examples will be presented for: (1)Improvement of optical resolution by confocal laser scanning 4Pi-microscopy (limit of the present device at Kirchhhoff-Institute about 100 nm axially using 2-Photon infrared excitation) and application to the analysis of membrane complexes and DNA replication factories. (2) Improvement of size resolution by Spatially Modulated Illumination (SMI) Microscopy (resolution of axial object extension down to few tens of nm using 1-Photon visible range laser excitation) and its application to the nanosizing of fluorescence labelled membrane complexes; of replication and transcription factories; and of specific gene domains. (3) Improvement of topological resolution by Spectral Precision Distance Microscopy of multispectrally labelled targets (3D distance resolution of few tens of nm) and its application in the analysis of specific gene domains.