Regensburg 2007 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 16: Poster Session I
BP 16.12: Poster
Dienstag, 27. März 2007, 17:00–19:30, Poster D
Nanotomography of Human Bone Based on Scanning Probe Microscopy — •Stephanie Röper1, Christian Dietz1, Sabine Scherdel1, Anke Bernstein2, Nicolaus Rehse1, and Robert Magerle1 — 1Chemische Physik, TU Chemnitz, D-09107 Chemnitz — 2Experimentelle Orthopädie, Martin-Luther-Universität Halle-Wittenberg, D-06097 Halle/Saale
Natural materials such as bone and teeth are nanocomposites of proteins and minerals, which exhibit a complex hierarchical structure ranging from macroscopic to molecular length scales. Scanning probe microscopy (SPM) based Nanotomography is a novel approach to image these materials. We focus on human bone which is first embedded in a methacrylate resin and then and then sectioned with the use of a microtome. For SPM based Nanotomography the specimen is ablated layer-by-layer by wet chemical etching and imaged with tapping mode scanning force microscopy after each etching step. From the resulting series of images the three-dimensional structure is reconstructed. The etching and imaging is done in-situ in a liquid cell of an SPM connected to reservoirs of etchants and water for flushing after each etching step. The flow of the different liquids is controlled with computer controlled valves which allow for an automated etching and measuring protocol. We will present first results of volume images of human bone and discuss our concepts for adjusting the imaging parameters to maintain a good imaging quality.