Regensburg 2007 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 16: Poster Session I
BP 16.26: Poster
Dienstag, 27. März 2007, 17:00–19:30, Poster D
Biofilm adsorption on structured substrates — •Hubert Mantz1, Christoph Gilow1, Anthony Quinn1, Karin Jacobs1, Markus Bellion2, and Ludger Santen2 — 1Saarland University, Experimental Physics, D-66041 Saarbrücken, Germany — 2Saarland University, Theoretical Physics, D-66041 Saarbrücken, Germany
The aim of this experimental study is to shed light on the mechanism of biofilm adsorption. The behavior of biomolecules at surfaces plays a fundamental role in a number of areas (e.g. the interaction between implant materials and human tissue) as structure of a protein is often closely related to its function.
Among a number of other factors, the complex process of protein adsorption is determined by surface properties. As a model system, a combination of structured substrates was used to isolate the contribution of different parameters (chemical composition, roughness, surface charge ...).
The adsorption kinetics of some proteins (amylase, lysozyme, albumin), as measured by in situ-ellipsometry have been compared to other techniques such as surface plasmon resonance and surface probe microscopy measurements.
The results have also been compared to Monte Carlo simulations, assuming conformational changes of the proteins with increasing surface coverage. Our results will be useful for the design of special (non-) adhesive biointerfaces.