Regensburg 2007 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 16: Poster Session I
BP 16.46: Poster
Dienstag, 27. März 2007, 17:00–19:30, Poster D
Cooperativity of Integrin-mediated Adhesion on Nanopatterned Substrates — •Christine Selhuber1,2, Thorsten Erdmann3, Ulrich Schwarz3, Horst Kessler4, and Joachim Spatz1,2 — 1Universität Heidelberg, Physikalisch-Chemisches Institut — 2Max-Planck-Institut für Metallforschung, Abteilung "Neue Materialien und Biophysik" — 3Universität Heidelberg, BIOMS — 4Technische Universität München, Department Chemie
Surfaces of defined adhesion properties are required for a physical and quantitative understanding of cell adhesion in vivo. In this work, biofunctional nanopatterns are employed, which allow adhesion ligands to be positioned in a quasi-hexagonal lattice. Such nanopatterns are used to investigate integrin-mediated cell adhesion, which is a highly complex biological process and essential for numerous cell functions. With nanopatterns the distance between adjacent single integrin binding sites is precisely defined. Recent cell culture experiments have revealed that this distance strongly affects cell adhesion and the formation of adhesion clusters, known as focal contacts. To quantify the adhesion cluster formation for different integrin binding site spacings, cell adhesion forces were studied using atomic force microscopy (AFM). The experiments demonstrate that an integrin binding site spacing of 70 nm and more prevents the cooperative formation of early adhesion clusters in initial adhesion. In long-term adhesion studies, after several hours of cell adhesion, it turned out that focal contact formation cooperatively increases the local adhesion strength. The obtained results were related to theoretical models on adhesion cluster stability.