Regensburg 2007 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 26: Poster Session II

BP 26.39: Poster

Donnerstag, 29. März 2007, 17:00–19:30, Poster B

Nanoengineered Polymer Capsules: Tools for Controlled delivery and Site Specific Manipulation — •Raghavendra Palankar, Yannic Ramaye, Sebastian Springer, and Mathias Winterhalter — IUB-Bremen, Campusring 1, 28725 Bremen

Hollow nanometer-sized containers are of increasing interest in nanotechnology, since they can protect proteins, enzymes or drugs from hostile surroundings and provide an optimal microenvironment. Here we report on functionalized nanocapsules as intracellular reporters providing a new tool in cell biology. Cell active molecules, hormones, enzymes or reporter molecules may be hidden from the outside, protected against chemical and biological degradation, targeted to specific compartments inside a cell and released in a controlled manner. To improve the separation of free from encapsulated material we use magnetic liposomes. In a further series we prepared hydrophobic superparamagnetic nanoparticles and entrapped then in the liposomal bilayer. This technique bypasses the step of gel filtration. Further, these magnetoliposomes are coated with alternating polymer polyelectrolyte layers, resulting in magnetoliposome capsules. These capsules are introduced into CHO or Vero cells by either electropermeabilization or microinjection.