Regensburg 2007 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 26: Poster Session II
BP 26.9: Poster
Donnerstag, 29. März 2007, 17:00–19:30, Poster B
RNA Unzipping in Nanopores Driven by Variable Forces — •Thomas Schötz1, Ralf Bundschuh2, and Ulrich Gerland3 — 1Arnold Sommerfeld Center for Theoretical Physics (ASC) and Center for Nanoscience (CenS), LMU München, Germany — 2Department of Physics, The Ohio State University, Columbus, U.S.A. — 3Institute for Theoretical Physics, University of Cologne, Köln, Germany
We study a theoretical model for voltage-driven translocation of structured RNA molecules through nanopores so narrow that only single strands can pass through. We focus on the sequence-dependent unzipping behaviour when the applied voltage is increased at a constant loading rate (voltage ramp), as in recent experiments with a single DNA-hairpin [1]. In order to describe the simultaneous, coupled dynamics of translocation and of the base-pairing patterns on each side of the pore, we apply a kinetic Monte Carlo scheme. Within this model, we determine the distribution of translocation times for different loading rates and sequences.
[1] Mathé, J., H. Visram, V. Viasnoff, Y. Rabin, and A. Meller (2004) Nanopore Unzipping of Individual DNA Hairpin Molecules. Biophys. J. 87, 3205–3212.