Regensburg 2007 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 9: Regulation and Signaling
BP 9.9: Vortrag
Dienstag, 27. März 2007, 12:15–12:30, H43
Analysis of bacterial gene regulatory networks by time-lapse fluorescence microscopy — •Judith Leierseder1, Georg Fritz1, Kirsten Jung2, and Joachim Rädler1 — 1Department für Physik, Ludwig-Maximilians-Universität München — 2Department Biologie I, Ludwig-Maximilians-Universität München
The vision of artificial genetic circuits with well defined response functions to external signals requires a quantitative understanding and description of the function of gene regulatory networks. Gene expression is, however, not only determined by the mere network structure, but also influenced by stochastic effects that lead to significant cell to cell variations. The single cell studies that are thus required to resolve these variations are greatly facilitated by the use of the green fluorescent protein (GFP) as network output marker. Using semi-automated time-lapse fluorescence microscopy combined with quantitative image processing we measured expression kinetics for many single bacterial cells. For our model system, the pBAD/AraC module in Escherichia coli, we analyzed the response following different induction concentrations and compared the kinetics to a simple theoretical gene expression model of the network.