Regensburg 2007 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 26: Organic, Polymeric, and Biomolecular Films II
O 26.4: Vortrag
Dienstag, 27. März 2007, 12:00–12:15, H42
Functional immobilization of proteins on solid substrates: An approach to protein chip nanotechnologies — •Andrey Turchanin1, Ali Tinazli2, Mohamed El-Desawy1, Robert Tampé2, and Armin Gölzhäuser1 — 1Physics of Supramolecular Systems, Department of Physics, University of Bielefeld, D-33615 Bielefeld — 2Institute of Biochemistry, Johann Wolfgang Goethe-University, D-60438 Frankfurt am Main
To study protein function and interaction, there is a high demand for specific, stable, locally immobilized, and functional protein arrays on solid substrates. To implement this, we propose a protein chip approach based on the combination of electron induced chemical nanolithography [1] with aromatic self-assembled monolayers (SAMs) and multivalent chelators [2] for high-affinity capturing of His6-tagged proteins. Templates for functional and switchable His-tagged protein architectures were created by electron beam induced changes in 4*-nitro-1,1*-biphenyl-4-thiol SAMs on gold placed in a protein repellent matrix. As a model system carboxy derivative EG3-tris-NTA and His6-tagged proteasome were studied. Different steps of the biochip templates fabrication were characterized by X-ray photoelectron spectroscopy. Their functionality was tested via in situ atomic force and fluorescence microscopy. We will present first results of functional protein arrays with about one micrometer lateral dimensions. [1] A. Gölzhäuser, W. Eck, W. Geyer, V. Stadler, T.Weimann, P. Hinze, M. Grunze, Adv. Mat. 13 (2001) 806. [2] A. Tinazli, J. Tang, R.Valiokas, S. Picuric, S. Lata, J. Piehler, B. Liedberg, R. Tampé, Chemistry 11 (2005) 5249.