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BP: Fachverband Biologische Physik

BP 7: Posters I

BP 7.10: Poster

Monday, February 25, 2008, 17:00–19:30, Poster A

Nanoengineered Polymer Capsules: Tools for Controlled delivery and Site Specific Manipulation — •Raghavendra Palankar¹, Oliver Kreft¹, Andre Skirtach¹, Yannic Ramaye¹, Margorzata Garstka², Gleb B. Sukhorukov², Sebastian Springer², and Mathias Winterhalter¹ — ¹Jacobs University Bremen — ²Max-Planck Institut Golm

Hollow nanometer-sized containers are of increasing interest in nanotechnology, since they can protect proteins, enzymes or drugs from hostile surroundings and provide an optimal microenvironment. Here we report on functionalized nanocapsules as intracellular reporters providing a new tool in cell biology. Cell active molecules, hormones, enzymes or reporter molecules may be hidden from the outside, protected against chemical and biological degradation, targeted to specific compartments inside a cell and released in a controlled manner. For example we loaded capsules with antigenic peptides and inject the capsule with electroporation. We describe here the laser-triggered release of peptides into the interior of a cell which is followed by their binding to MHC class I molecules, and the subsequent movement of the peptide-class I complex to the plasma membrane.

Sukhorukov GB et al. Multifunctionalized Polymer Microcapsules: Novel Tools for Biological and Pharmacological Applications. Small 3 (2007) 944-55.