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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 9: POSTERS Single Molecules, Biopolymers, Membranes

CPP 9.4: Poster

Monday, February 25, 2008, 16:45–19:00, Poster A

New NIR fluorophores for single molecule microscopy — Georg M. Fischer, Martin Winterhalder, Magnus Isomäki-Krondahl, Andreas Zumbusch, and •M. Yavuz Yüce — Department Chemie, Universität Konstanz, Fach M722, D-78457 Konstanz

Excitation in the near infrared (NIR) geneally leads to lower background signal in single molecule experiments. For this reason, strongly fluorescent chromophores which absorb and emit in the NIR are highly desirable for such applications. According to the energy gap law, however, most fluorophores will only exhibit very weak NIR fluorescence emission. Here we present a new class of dyes based on diketopyrrolopyrroles. At absorption wavelengths of up to 800 nm, these dyes still exhibit fluorescence quantum yields of more than 50%. We have characterized a broad variety of derivatives both on the ensemble and on the single molecule level.