Berlin 2008 – wissenschaftliches Programm
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DF: Fachverband Dielektrische Festkörper
DF 9: Poster
DF 9.11: Poster
Dienstag, 26. Februar 2008, 15:00–18:00, Poster G
Confocal Raman and Fluorescence Lifetime Imaging (FLIM) Microscope — •Philipp Schreier1, Lothar Kador1, Deliani Lovera2, and Volker Altstädt2 — 1University of Bayreuth, Institute of Physics and BIMF, 95440 Bayreuth — 2University of Bayreuth, Department of Polymer Engineering, 95440 Bayreuth
We present a custom-built set-up capable of performing three-dimensional confocal Raman and fluorescence lifetime imaging (FLIM) microscopy. 3-d Raman measurements were performed on spider silk fibers and electro-spun electret fibers which were prepared from miscible and non-miscible polymer mixtures. In both cases of the electret materials, the compositions of the fibers were homogeneous on the micrometer scale. For the FLIM experiments the cw laser is modulated with an acousto-optic modulator in the MHz regime, and the phase shift of the fluorescence light with respect to the excitation is measured. In this way, fluorescence lifetimes in the nanosecond range can be obtained. First data of, e. g., paper samples stained with different fluorescent dyes are presented.