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Darmstadt 2008 – wissenschaftliches Programm

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MO: Fachverband Molekülphysik

MO 16: Biomoleküle

MO 16.11: Poster

Dienstag, 11. März 2008, 14:00–19:00, Poster C1

Fluorescence correlation spectroscopy on flavoproteins — •Christian Brock1, Florian Spreitler2, Astrid Pelzmann3, Ortwin Meyer4, and Jürgen Köhler51Lehrstuhl für Experimentalphysik 4, Universität Bayreuth — 2Lehrstuhl für Experimentalphysik 4, Universität Bayreuth — 3Lehrstuhl für Mikrobiologie, Universität Bayreuth — 4Lehrstuhl für Mikrobiologie, Universität Bayreuth — 5Lehrstuhl für Experimentalphysik 4, Universität Bayreuth

We use fluorescence correlation spectroscopy to investigate the binding properties of CO-dehydrogenase, a flavoenzyme that plays an important role in the respiratory chain of the CO-oxidizing bacterium Oligotropha carboxidovorans. CO-dehydrogenase is labeled with fluorescein, and the change of its diffusion-coefficient upon binding to a lipid vesicle is monitored. This experiment will be repeated with modified versions of the enzyme, lacking certain functional groups that are supposed to be responsible for binding. Furthermore, triplet kinetics of FAD and riboflavin have been analyzed.

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