Darmstadt 2008 – scientific programme
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MO: Fachverband Molekülphysik
MO 18: Femtosekundenspektroskopie
MO 18.9: Poster
Tuesday, March 11, 2008, 14:00–19:00, Poster C1
Detection of C-Deuterium compounds by CARS microscopy — •Gero Bergner1,3, Denis Akimov1, Michael Schmitt1,2, Sebastian Schlücker3, and Jürgen Popp1,2 — 1Institut für Photonische Technologien, Albert-Einstein-Straße 9, 07745 Jena — 2Institut für Physikalische Chemie, Lessingstraße 10, 07743 Jena — 3Institut für Physikalische Chemie, Am Hubland, 97074 Würzburg
Coherent anti-Stokes Raman scattering (CARS) microscopy is a powerful imaging technique with chemical specificity. In particular, it provides vibrational contrast without need of external labelling. Here, we present first steps to establish CARS as a new method for drug localization in living cells. Chemical contrast in the images is obtained by probing the C-D stretch vibration of internally labelled (Deuterium-substituted) compounds. For determining the ratio of Raman-resonant signal to non-Raman-resonant background, both C-D and C-H species of the same compound are investigated. First results for systematically determining the detection limit of this methodology are presented.