Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 13: Cell Migration
BP 13.3: Vortrag
Mittwoch, 25. März 2009, 11:15–11:30, ZEU 260
Mimicking Cellular Environments: Cells on elastic nanopatterned substrates — •Ilia Louban1,2, Roberto Fiammengo1,2, and Joachim Spatz1,2 — 1MPI for Metals Research, Dept. of New Materials & Biosystems; Heisenbergstr. 3, D-70569 Stuttgart — 2Univ. of Heidelberg, Dept. of Biophys. Chemistry; INF 253, D-69120 Heidelberg
The last years, hydrogels based on poly(ethylene glycol) diacrylate (PEG-DA) have been developed to serve as synthetic extracellular matrix analogues with adjustable mechanical and biochemical properties. Their Young's moduli (E) span more than four orders of magnitude (0,6kPa<E<6MPa). Since PEG-DA features protein and consequentially cell repellent properties, the hydrogel surface has to be modified to provide bioactivity. Extended gold nanoparticle arrays, manufactured by block copolymer micellar nanolithography, could be transferred to the hydrogel surface providing single anchor points for biofunctionalization. The interparticle distance (30nm<ΔL<200nm) on the substrate can be varied independently from its rigidity. To promote integrin mediated cell adhesion of rat embryonic fibroblasts, gold nanoparticles were functionalized with a cRGDfK peptide specific for αVβ3 integrin. The effect of variation of substrate compliance and interparticle distance, tuned at the same time, was investigated. Our experiments reveals a significant decrease in cell spreading area on soft substrates (E<10kPa) and substrate with high interparticle distance (ΔL>70nm) after 6, 12 and 24 hours of adhesion respectively. Additionally we performed atomic force spectroscopy to quantify cellular adhesion to these surfaces.