Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 19: Cell Adhesion
BP 19.4: Vortrag
Donnerstag, 26. März 2009, 11:30–11:45, ZEU 260
Dissecting the Impact of Matrix Anchorage and Elasticity in Cell Adhesion — •Tilo Pompe1, Stefan Glorius1, Thomas Bischoff1, Ina Uhlmann1, Martin Kaufmann1, Sebastian Brenner2, and Carsten Werner1 — 1Leibniz-Institut für Polymerforschung, Dresden, Germany — 2Universitätsklinik C.G. Carus, Dresden, Germany
Extracellular matrices determine cellular fate decisions through the regulation of intracellular force and stress. It was anticipated that matrix stiffness and ligand anchorage would have distinct effects on the signalling cascades involved. We now can show how defined non-covalent anchorage of adhesion ligands onto elastic substrates allows the dissection of intracellular adhesion signalling pathways. Fourier transform traction cytometry proved the regulation of cell traction forces by the strength of the non-covalent anchorage of extracellular matrix ligands to the substrate. Using these constrained traction force levels the strain energy exerted by the cell on the substrate was quantitatively described by treating the cell as active force dipoles. Moreover matrix stiffness could be demonstrated to be the dominant exogenous signal of the global mechanical balance in cell adhesion. Besides the decoupling of biophysical signals biochemical signals like phosphorylation of the adhesion signalling protein FAK were distinctively controlled by matrix elasticity but not by varied receptor forces. Furthermore, using the net traction dipole moment of adherent cells our approach revealed a basis for a generalised biophysical treatment of extracellular mechanical signals in cell adhesion.