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BP: Fachverband Biologische Physik

BP 7: Poster I

BP 7.22: Poster

Montag, 23. März 2009, 17:45–20:00, P3

Optical Tweezer: A system for tracking several beads incorporated in the keratin cytoskeleton of pancreatic carcinoma cells — •Tobias Paust¹, Alexander Schmatulla¹, Ulla Nolte¹, Michael Beil², and Othmar Marti¹ — ¹Institute of Experimental Physics, University of Ulm, D-89069 Ulm, Germany — ²Internal Medicine I, University of Ulm, D-89069 Ulm, Germany

The biophysical and viscoelastic properties of the keratin cytoskeleton have an effect on the ability of migration of the pancreatic carcinoma cells in the extracellular environment. Therefore this project handles about the investigation and characterization of these viscoelastic properties of the keratine networks. With the extraction of the cytoplasmatic elements out of the cell the keratin networks are isolated. This ensures that there are no biochemical interrupts of interactions between the network elements. The method of measurement implies the trapping of a polystyrene sphere and the subsequent movement by the laser light of an optical tweezer. In contact with the cytoskeleton it is possible to determine the mechanical properties of the cytoskeleton by analyzing auto- and crosscorrelation of the trapped bead. A high speed camera was incorporated to measure this spatial response by tracking many particles simultaneously with a time resolution better than 1ms. The first measurements depict the dependency of the response of multiple nanometric spheres on the time variable forces of the cytoskeleton.