Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Poster I
BP 7.45: Poster
Montag, 23. März 2009, 17:45–20:00, P3
NIR SERS hybrid probes for in vitro and in vivo bioanalytics — •Andrea Matschulat1,2, Ilona Dörfel1, Franziska Emmerling1, and Janina Kneipp1,2 — 1Federal Institute of Materialsresearch and Testing (BAM) — 2Department of Chemistry, Humboldt University, 12489 Berlin, Germany
Raman Spectroscopy, a method with many applications ranging from condensed matter physics to bioanalytical chemistry, offers several advantages such as the rapid and non-destructive study of vibrational fingerprints of chemical and biological compounds. With SERS, scattering efficiencies can be enlarged by a factor of ∼ 106 in bulk samples. This versatile and selective technique provides more sensitive detection, accompanied by high spatial resolution due to local optical near-fields generated by noble metal nanostructures. Our work is concerned with the construction and characterization of Au and Ag nanoparticles whose unique plasmonic properties are tuned for the sensitive NIR-SERS probing of complex biosamples. In our studies, we were successful in detecting spectral fingerprints of various Raman reporter species (DTNB, NT, MBA and PATP) that contrast strongly with commonly used reporter fluorophores due to their larger SERS cross sections. Further, their utilization in multiplex approaches under physiological conditions enables the identification of different types of labelled SERS probes. In first in vitro experiments, we have introduced SERS hybrid probes, with which intrinsic information of pollen extracts can be obtained. The role of BSA as a stabilizing agent for nanoparticles and linker for multiple analytes in both will also be discussed.