Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Poster I
BP 7.48: Poster
Montag, 23. März 2009, 17:45–20:00, P3
Deposition of engineered nanoparticles on human lung cells via the air liquid interface — •Andreas Comouth1,2, Sonja Muelhopt2, Harald Saathoff1, Daniel Rzesanke1, Alicja Panas3, Carsten Weiss3, Hanns-Rudolf Paur2, Silvia Diabate3, and Thomas Leisner1 — 11Institute for Meteorology and Climate Research, Forschungszentrum Karlsruhe, Germany — 22Institute of Technical Chemistry, Thermal Waste Treatment Division, Forschungszentrum Karlsruhe, Germany — 3Institute of Toxicology and Genetics, Forschungszentrum Karlsruhe, Germany
Epidemiological studies show a correlation between the concentration of ultrafine particles in the atmosphere and the rate of mortality and morbidity due to respiratory and cardiovascular disease. In order to get quantitative information about the lung toxicity of engineered airborne nanoparticles an in vitro exposure system has been build up and lung specific bioassays have been developed. Unlike submers exposure this set up is more realistic due to the deposition at the air liquid interface of lung cells as it happens in vivo. Further this method enables reproducible deposition conditions by in situ monitoring of particle size distribution and concentration via scanning mobility particle sizing (SMPS) as well as mass dose determination by a quartz crystal microbalance. After exposure at the air liquid interface the cells are analyzed to measure the biological responses such as viability, inflammatory or oxidative stress. In this way it is possible to study the influence of particle properties such as surface area, particle coatings as well as primary particle size and agglomerate size on lung toxicity.