Dresden 2009 – wissenschaftliches Programm
CPP 16.15: Poster
Dienstag, 24. März 2009, 14:00–16:30, P3
The influence of substrates on protein adsorption: adsorption kinetics studies and structural investigations. — •Hendrik Hähl, Hubert Mantz, and Karin Jacobs — Saarland University, Experimental Physics, D-66041 Saarbruecken, Germany
Surfaces that come into contact with an aqueous solution of proteins will be immediately covered with a thin protein layer. We seek to better understand the driving forces of this process. Concentrating on the influence of the substrate on the adsorption, the surface chemistry has been the focus of many studies. Recent studies have shown that, next to the chemical composition of the uppermost surface layer, also the substrate’s composition influences the adsorption kinetics of proteins such as α-amylase and BSA [1, 2]. The experimental findings are corroborated by simulations assuming conformational or orientational changes of the protein molecules upon adsorption.
Thus, one also assumes different structures of the formed protein layers. To experimentally observe such differences, high resolution measurements of film thickness and density are needed. Therefore, we used surface plasmon resonance enhanced ellipsometry as well as neutron and X-ray reflectivity measurements for better film thickness and density data.
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