Dresden 2009 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 42: Poster Session II (Nanostructures at surfaces: arrays; Nanostructures at surfaces: Dots, particles, clusters; Nanostructures at surfaces: Other; Nanostructures at surfaces: Wires, tubes; Metal substrates: Adsorption of O and/or H; Metal substrates: Clean surfaces; Metal substrates: Adsorption of organic/bio moledules; Metal substrates: Solid-liquid interfaces; Metal substrates: Adsorption of inorganic molecules; Metal substrates: Epitaxy and growth; Heterogeneous catalysis; Surface chemical reactions; Ab-initio approaches to excitations in condensed matter; Organic, polymeric, biomolecular films– also with adsorbates; Particles and clusters)
O 42.116: Poster
Mittwoch, 25. März 2009, 17:45–20:30, P2
Nano-Mechanical Detection of (Bio-)Molecular Recognition — •K. Gruber1, C. Rohr1, J. Koeser2, U. Hubler2, T. Sobey3, F. C. Simmel3, and B. A. Hermann1 — 1Dept. of Physics / CeNS, LMU Munich and WMI, 85748 Garching, Germany — 2Concentris GmbH, 4012 Basel, Switzerland — 3Dept. of Physics, TU Munich, 85748 Garching, Germany
Based on the development of the atomic force microscope, the cantilever array technique monitors physical, chemical and biochemical processes taking place on the surface of wafer-thin and extremely sensitive silicon springs. These reactions can be measured directly without fluorescent or radioactive labels, and can be followed in real-time allowing analysis of reaction dynamics and determination of e.g. kinetic constants. The goal is to understand the chemical transduction process of a (bio-)molecular key-lock-recognition in a deflection signal by performing well defined experiments.
The hybridization signals of two 70% to 80% homologous single-stranded DNA sequences could be clearly distinguished. Each half of one cantilever array was functionalized with one of the two DNA strands. Hence, only those cantilever that bear the exact complementary to a matching single strand, offered in the sample solution, show deflection. The experiment could be successfully reproduced for several independently functionalized cantilever arrays. As one possible application, we demonstrated that a thrombin catching DNA-aptamer[1] could be successfully bound onto a cantilever surface.
[1] F. C. Simmel et al., Angew. Chem. Int. Ed. 2004 43, 3549 (2004)